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Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon agroinfiltration. The first method details how to transform bioluminescent AgroLux to carry binary plasmids of interests. Then, a simple agroinfiltration assay for in planta imaging of bioluminescence signals is presented. AgroLux assays will increase our understanding of plant-Agrobacterium interactions and plant immunity and improve molecular farming.

Original publication

DOI

10.1007/978-1-0716-2241-4_15

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2022

Volume

2480

Pages

285 - 293

Keywords

AgroLux, Agrobacterium tumefaciens, Agroinfiltration, Bacterial bioluminescence, Nicotiana benthamiana, Agrobacterium tumefaciens, Plant Immunity, Plasmids, Tobacco