Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We recently developed a simple method called "agromonas" which facilitates the detection of Pseudomonas syringae growth in agroinfiltrated leaves expressing genes of interest. This protocol consists of agroinfiltration followed by inoculation of Pseudomonas syringae by either infiltration or spray inoculation. Next, bacterial growth is measured a few days later by plating leaf extracts out on P. syringae-selective medium. In this protocol, we describe all the steps required for agroinfiltration, inoculation of P. syringae by both injection and spray infection and explain how to quantify in planta live bacteria.

Original publication

DOI

10.1007/978-1-0716-2079-3_20

Type

Chapter

Publication Date

2022

Volume

2447

Pages

247 - 259

Keywords

Agrobacterium tumefaciens, Agroinfiltration, Disease assay, Infiltration, Pseudomonas syringae, Spray-inoculation, Plant Leaves, Pseudomonas syringae