Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

This paper describes the use of the immunoperoxidase technique for the screening of rat hybridoma culture supernatants on tissue sections. By combining the avidin-biotin system with mouse monoclonal antibodies specific to different rat immunoglobulin isotypes, it is possible to resolve the specificity patterns of complex mixtures of monoclonal antibodies from uncloned culture wells. This strategy is particularly useful in the derivation of monoclonal antibodies to cell surface antigens.

Type

Journal article

Journal

J Immunol Methods

Publication Date

27/04/1984

Volume

69

Pages

207 - 214

Keywords

Animals, Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Antibody Specificity, Antigens, Surface, Histocytochemistry, Humans, Hybridomas, Immunoenzyme Techniques, Immunoglobulin Allotypes, Immunoglobulin G, Immunoglobulin kappa-Chains, Mice, Palatine Tonsil, Rats