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Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. For two well-defined Pol II-transcribed genes, CYC1 and PMA1, we demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol II, following mRNA 3' end processing. Similarly, Pol I termination relies on prior Rnt1p cleavage at the 3' end of the pre-rRNA 35S transcript. This is followed by the combined actions of Rat1p and Sen1p to degrade the Pol I-associated nascent transcript that consequently promote termination in the downstream rDNA spacer sequence. Our data suggest that the previously defined in vitro Pol I termination mechanism involving the action of the Reb1p DNA-binding factor to "road-block" Pol I transcription close to the termination region may have overlooked more complex in vivo molecular processes.

Original publication

DOI

10.1101/gad.463408

Type

Journal article

Journal

Genes Dev

Publication Date

15/04/2008

Volume

22

Pages

1082 - 1092

Keywords

Cytochromes c, DNA Helicases, DNA, Ribosomal, Exoribonucleases, Fungal Proteins, Models, Biological, Proton-Translocating ATPases, RNA Helicases, RNA Polymerase I, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Terminator Regions, Genetic, Transcription, Genetic