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ADP-ribosylation is a widespread reversible chemical modification of macromolecular targets. Protein ADP-ribosylation has been widely studied and plays a vital role in the regulation of several biological processes. In recent years there has been increasing interest in alternative ADP-ribosylation targets such as nucleic acids-DNA and RNA. Here we report different methods to detect ADP-ribosylation of RNA substrates.

Original publication

DOI

10.1007/978-1-0716-1374-0_15

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2021

Volume

2298

Pages

231 - 243

Keywords

ADP-ribosylation, PARPs, RNA, TRPT1