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Bunyamwera virus and snowshoe have virus (family Bunyaviridae) were passaged up to six times through mosquito cells in culture and the resultant viruses were compared to the input, mammalian cell-passed virus using monoclonal antibodies raised against the input virus. The mosquito cell-adapted virus population consisted of mutants which were better adapted to replication in the new host than was the input mammalian cell-passed virus and were differentially susceptible to neutralization by antibody.

Original publication

DOI

10.1099/0022-1317-67-12-2803

Type

Journal article

Journal

J Gen Virol

Publication Date

12/1986

Volume

67 ( Pt 12)

Pages

2803 - 2806

Keywords

Aedes, Animals, Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Bunyamwera virus, Bunyaviridae, Cell Line, Cercopithecus aethiops, Epitopes, Genes, Viral, Mutation, Neutralization Tests, Viral Envelope Proteins, Viral Plaque Assay, Virus Replication