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The effect of internal Cl- on the K-ATP channel of pancreatic beta-cells was examined. Reducing Cl- from 140mM to 14mM potentiated channel activity (NPO) approximately 4.5 fold in 60% of patches but was without effect in 40% of patches. The Ki for channel inhibition by ATP was not changed. The inhibitory effect of Cl- interacted with the stimulatory action of MgGDP. In 140mM Cl-, 1mM MgGDP increased NPO in Cl(-)-sensitive patches only: when NPO was potentiated by 14mM Cl-, no further increase was produced by MgGDP. These observations suggest that MgGDP and low Cl- solution may increase channel activity via a similar mechanism.


Journal article


Pflugers Arch

Publication Date





194 - 196


Adenosine Triphosphate, Animals, Cells, Cultured, Chlorides, Intracellular Membranes, Islets of Langerhans, Membrane Potentials, Mice, Patch-Clamp Techniques, Potassium Channels