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Subventricular zone (SVZ) cells emigrate toward brain injury but relatively few survive. Thus, if they are to be used for repair, ex vivo expansion and autologous transplantation of SVZ cells may be necessary. Since it is unclear how brain injury alters SVZ cell culture, we studied neurosphere formation, differentiation, and migration, after cortical lesions. The number of neurosphere forming cells from lesioned mice was comparable to controls. Also, the proportion of astrocytes and neurons generated in vitro remained unchanged after cortical lesions. Cell emigration from neurospheres was characterized by increased cell-cell contact after injury in adults and neonates. However, neither molecules implicated in SVZ migration nor the extent of migration changed after injury. Thus, neurospheres can be successfully cultured after extensive brain damage, and they are remarkably stable in vitro, suggesting suitability for ex vivo expansion and autologous transplantation.

Original publication




Journal article



Publication Date





717 - 725


Animals, Animals, Newborn, Brain Injuries, Cell Count, Cell Death, Cell Differentiation, Cell Movement, Cell Proliferation, Cerebral Ventricles, Immunohistochemistry, In Vitro Techniques, Integrin alpha6, Integrin beta1, Male, Neural Cell Adhesion Molecules, Neuroglia, Neurons, Stem Cells, Time Factors