Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.

Original publication

DOI

10.1038/nmeth.1206

Type

Journal article

Journal

Nat Methods

Publication Date

05/2008

Volume

5

Pages

397 - 399

Keywords

Animals, Carcinoembryonic Antigen, Cell Survival, Cells, Cells, Cultured, Electrophoresis, Agar Gel, Fluorescent Dyes, Image Processing, Computer-Assisted, Ligands, Nanotechnology, Polyethylene Glycols, Quantum Dots, Rats, Receptors, Cell Surface, Receptors, Glutamate, Staining and Labeling, Streptavidin, Synapses