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We report for the first time on pore-suspending lipid bilayers, which we call micro-black lipid membranes (micro-BLMs), based on a highly ordered macroporous silicon array. Micro-BLMs were established by first functionalizing the backside porous silicon surface with gold and then chemisorbing 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol followed by spreading 1,2-diphytanoyl-sn-glycero-3-phosphocholine dissolved in n-decane. Impedance spectroscopy revealed the formation of single lipid bilayers confirmed by a mean specific capacitance of 0.6 +/- 0.2 microF/cm2. Membrane resistances were in the G omega-regime and beyond. The potential of the system for single channel recordings was demonstrated by inserting the transmembrane domain of the HIV-1 accessory peptide Vpu(1-32), which forms helix bundles with characteristic opening states. We elucidated different amilorides as potential drugs to inhibit channel activity of Vpu.

Original publication




Journal article


J Am Chem Soc

Publication Date





16267 - 16274


Amiloride, Electric Impedance, Human Immunodeficiency Virus Proteins, Ion Channels, Lipid Bilayers, Membrane Potentials, Membranes, Artificial, Microscopy, Electron, Scanning, Peptide Fragments, Protein Structure, Tertiary, Silicon, Viral Regulatory and Accessory Proteins