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X-ray structures are known for three members of the Major Facilitator Superfamily (MFS) of membrane transporter proteins, thus enabling the use of homology modeling to extrapolate to other MFS members. However, before employing such models for, e.g., mutational or docking studies, it is essential to develop a measure of their quality. To aid development of such metrics, two disparate MFS members (NupG and GLUT1) have been modeled. In addition, control models were created with shuffled sequences, to mimic poor quality homology models. These models and the template crystal structures have been examined in terms of both static and dynamic indicators of structural quality. Comparison of the behavior of modeled structures with the crystal structures in molecular dynamics simulations provided a metric for model quality. Docking of the inhibitor forskolin to GLUT1 and to a control model revealed significant differences, indicating that we may identify accurate models despite low sequence identity between target sequences and templates.

Original publication

DOI

10.1529/biophysj.106.093971

Type

Journal article

Journal

Biophys J

Publication Date

15/11/2006

Volume

91

Pages

L84 - L86

Keywords

Binding Sites, Computer Simulation, Membrane Transport Proteins, Models, Chemical, Models, Molecular, Protein Binding, Protein Conformation, Sequence Analysis, Protein