Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Potassium channels have been studied intensively in terms of the relationship between molecular structure and physiological function. They provide an opportunity to integrate structural and computational studies in order to arrive at an atomic resolution description of mechanism. We review recent progress in K channel structural studies, focussing on the bacterial channel KcsA. Structural studies can be extended via use of computational (i.e. molecular simulation) approaches in order to provide a perspective on aspects of channel function such as permeation, selectivity, block and gating. Results from molecular dynamics simulations are shown to be in good agreement with recent structural studies of KcsA in terms of the interactions of K(+) ions with binding sites within the selectivity filter of the channel, and in revealing the importance of filter flexibility in channel function. We discuss how the KcsA structure may be used as a template for developing structural models of other families of K channels. Progress in this area is explored via two examples: inward rectifier (Kir) and voltage-gated (Kv) potassium channels. A brief account of structural studies of ancillary domains and subunits of K channels is provided.

Type

Journal article

Journal

Biochim Biophys Acta

Publication Date

11/10/2002

Volume

1565

Pages

294 - 307

Keywords

Amino Acid Sequence, Animals, Bacterial Proteins, Binding Sites, Cations, Monovalent, Computer Simulation, Crystallography, X-Ray, Humans, Membrane Proteins, Models, Molecular, Molecular Sequence Data, Molecular Structure, Potassium Channels, Potassium Channels, Inwardly Rectifying, Potassium Channels, Voltage-Gated, Protein Structure, Tertiary, Sequence Alignment, Structure-Activity Relationship