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A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation during meiosis.

Original publication




Journal article


Nucleic Acids Res

Publication Date





1866 - 1873


DNA Helicases, DNA-Binding Proteins, Endonucleases, Genetic Complementation Test, Holliday Junction Resolvases, Humans, Meiosis, Mutagens, Mutation, Phenotype, Recombination, Genetic, Schizosaccharomyces, Schizosaccharomyces pombe Proteins