Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Courtship can be defined as behavioral interactions between males and females, the evolutionary objective of which is copulation and the ultimate perpetuation of the species. This protocol allows determination of two aspects of courtship in Drosophila: to assess whether there is a deficiency in mating frequency and, if this is the case, to resolve the nature of the specific problem. The first part of the approach provides a simple, objective, high-throughput strategy that is ideal for determining whether a specific strain has any courtship defect. Any strain that mates at a frequency comparable to that of wild-type flies must be considered reasonably fit in an evolutionary sense. If a specific strain has an abnormal mating frequency, we are then interested in determining whether there is a specific courtship defect, as described in the second half of the protocol. This requires direct live observation or digital recording of courtship.

Original publication

DOI

10.1101/pdb.prot071647

Type

Journal article

Journal

Cold Spring Harb Protoc

Publication Date

01/11/2012

Volume

2012

Pages

1140 - 1145

Keywords

Animals, Courtship, Drosophila melanogaster, Entomology, Female, High-Throughput Screening Assays, Male, Sexual Behavior, Animal