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Neurotensin receptor type 1 (NTS1) is a G protein-coupled receptor (GPCR) that affects cellular responses by initiating a cascade of interactions through G proteins. The kinetic details for these interactions are not well-known. Here, NTS1-nanodisc-Gαs and Gαi1 interactions were studied. The binding affinities of Gαi1 and Gαs to NTS1 were directly measured by surface plasmon resonance (SPR) and determined to be 15±6 nM and 31±18 nM, respectively. This SPR configuration permits the kinetics of early events in signalling pathways to be explored and can be used to initiate descriptions of the GPCR interactome.

Original publication

DOI

10.1016/j.febslet.2014.10.043

Type

Journal article

Journal

FEBS Lett

Publication Date

20/12/2014

Volume

588

Pages

4701 - 4707

Keywords

Electron microscopy, G protein, G protein-coupled receptor, Nanodisc, Surface plasmon resonance, Cell Membrane, GTP-Binding Protein alpha Subunits, Gi-Go, GTP-Binding Protein alpha Subunits, Gs, Kinetics, Models, Molecular, Nanostructures, Protein Conformation, Receptors, Neurotensin, Signal Transduction