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In mitosis, animal cells lose their adhesion to the surrounding surfaces and become rounded. During mitotic exit, they reestablish these adhesions and at the same time physically contract and divide. How these competing processes are spatially segregated at the cell cortex remains mysterious. To address this question, we define the specific effector pathways used by RhoA and Rac1 in mitotic cells. We demonstrate that the MKlp1-CYK4 centralspindlin complex is a guanosine triphosphatase-activating protein (GAP) for Rac1 and not RhoA and that CYK4 negatively regulated Rac1 activity at the cell equator in anaphase. Cells expressing a CYK4 GAP mutant had defects in cytokinesis and showed elevated staining for the cell adhesion marker vinculin. These defects could be rescued by depletion of ARHGEF7 and p21-activated kinase, Rac1-specific effector proteins required for cell adhesion. Based on these findings, we propose that CYK4 GAP activity is required during anaphase to inhibit Rac1-dependent effector pathways associated with control of cell spreading and adhesion.

Original publication

DOI

10.1083/jcb.201204107

Type

Journal article

Journal

J Cell Biol

Publication Date

03/09/2012

Volume

198

Pages

865 - 880

Keywords

Anaphase, Cell Adhesion, Cell Line, Tumor, Cytokinesis, GTPase-Activating Proteins, Guanine Nucleotide Exchange Factors, HeLa Cells, Humans, Microtubule-Associated Proteins, Mitosis, Rho Guanine Nucleotide Exchange Factors, Signal Transduction, Vinculin, cdc42 GTP-Binding Protein, p21-Activated Kinases, rac1 GTP-Binding Protein, rhoA GTP-Binding Protein