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Ig-like transcript 4 (ILT4) (also known as leukocyte Ig-like receptor 2, CD85d, and LILRB2) is a cell surface receptor expressed mainly on myelomonocytic cells, whereas ILT2 (also known as leukocyte Ig-like receptor 1, CD85j, and LILRB1) is expressed on a wider range of immune cells including subsets of natural killer and T cells. Both ILTs contain immunoreceptor tyrosine-based inhibitory receptor motifs in their cytoplasmic tails that inhibit cellular responses by recruiting phosphatases such as SHP-1 (Src homology 2 domain containing tyrosine phosphatase 1). Although these ILTs have been shown to recognize a broad range of classical and nonclassical human MHC class I molecules (MHCIs), their precise binding properties remain controversial. We have used surface plasmon resonance to analyze the interaction of soluble forms of ILT4 and ILT2 with several MHCIs. Although the range of affinities measured was quite broad (Kd = 2-45 microM), some interesting differences were observed. ILT2 generally bound with a 2- to 3-fold higher affinity than ILT4 to the same MHCI. Furthermore, ILT2 and ILT4 bound to HLA-G with a 3- to 4-fold higher affinity than to classical MHCIs, suggesting that ILT/HLA-G recognition may play a dominant role in the regulation of natural killer, T, and myelomonocytic cell activation. Finally, we show that ILT2 and ILT4 effectively compete with CD8 for MHCI binding, raising the possibility that ILT2 modulates CD8+ T cell activation by blocking the CD8 binding as well as by recruiting inhibitory molecules through its immunoreceptor tyrosine-based inhibitory receptor motif.

Original publication

DOI

10.1073/pnas.1431057100

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

22/07/2003

Volume

100

Pages

8856 - 8861

Keywords

Amino Acid Sequence, Antigens, CD, Base Sequence, Binding Sites, Binding, Competitive, CD8 Antigens, CD8-Positive T-Lymphocytes, DNA, Complementary, HLA Antigens, HLA-G Antigens, Histocompatibility Antigens Class I, Humans, In Vitro Techniques, Killer Cells, Natural, Kinetics, Leukocyte Immunoglobulin-like Receptor B1, Membrane Glycoproteins, Models, Molecular, Molecular Sequence Data, Protein Conformation, Receptors, Immunologic, Recombinant Proteins, Sequence Homology, Amino Acid, Surface Plasmon Resonance