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In the absence of a suitable carbohydrate-based vaccine, outer membrane vesicle (OMV) vaccines have been used to disrupt outbreaks of serogroup B meningococcal disease for more than 20 years. Proteomic technology provides physical methods with the potential to assess the composition and consistency of these complex vaccines. 2-DE, combined with MS, were used to generate a proteome map of an OMV vaccine, developed to disrupt a long-running outbreak of group B disease in New Zealand. Seventy four spots from the protein map were identified including the outer membrane protein (OMP) antigens: PorA, PorB, RmpM and OpcA. Protein identification indicates that, in addition to OMPs, OMV vaccines contain periplasmic, membrane-associated and cytoplasmic proteins. 2-D-DIGE technology highlighted differences between preclinical development batches of vaccines from two different manufacturers.

Original publication

DOI

10.1002/pmic.200500821

Type

Journal article

Journal

Proteomics

Publication Date

06/2006

Volume

6

Pages

3400 - 3413

Keywords

Antigens, Bacterial, Bacterial Outer Membrane Proteins, Electrophoresis, Gel, Two-Dimensional, Humans, Mass Spectrometry, Meningococcal Vaccines, Neisseria meningitidis, Serogroup B, Proteomics