Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative alpha-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

Type

Conference paper

Publication Date

27/11/2003

Volume

555

Pages

170 - 175

Keywords

Bacterial Proteins, Base Sequence, Carrier Proteins, Circular Dichroism, Cloning, Molecular, DNA, Bacterial, DNA, Recombinant, Escherichia coli, Genes, Bacterial, Genetic Vectors, Helicobacter pylori, Membrane Proteins, Plasmids, Recombinant Proteins, Solubility