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We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

Type

Journal article

Journal

J Virol

Publication Date

11/1999

Volume

73

Pages

9679 - 9682

Keywords

Animals, Cell Line, DNA, Recombinant, Genetic Techniques, Humans, Influenza A virus, Plasmids, RNA, Viral, Transcription, Genetic, Transfection, Viral Proteins