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Influenza virus RNA-dependent RNA polymerase is a heterotrimeric complex of PB1, PB2, and PA. We show that the individually expressed PB2 subunit can be assembled with the coexpressed PB1-PA dimer in vitro into a transcriptionally active complex. Furthermore, we demonstrate that a model viral RNA promoter can bind to the PB1-PA dimer prior to assembly with PB2. Our results are consistent with a recently proposed model for the sequential assembly of viral RNA polymerase complex in which the PB1-PA dimeric complex and the PB2 monomer are transported into the nucleus separately and then assembled in the nucleus.

Original publication

DOI

10.1128/JVI.79.13.8669-8674.2005

Type

Journal article

Journal

J Virol

Publication Date

07/2005

Volume

79

Pages

8669 - 8674

Keywords

Cell Line, Dimerization, Humans, Influenza A virus, Models, Biological, Promoter Regions, Genetic, Protein Subunits, RNA Replicase, Transfection, Viral Proteins