Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the chromosomal DNAs ranged from 3.5 to 1.2 megabase pairs (Mb). By Southern hybridization with homologous gene probes, the chromosomal location of cellulase and laccase genes have been mapped. In addition, rDNA has been assigned to chromosomal bands using a heterologous gene probe. Genomic rearrangement is suggested in the commercial heterokaryon, as indicated by the presence of non-comigrating homologous chromosomes, identified by a number of probes for particular DNA sequences.

Type

Journal article

Journal

Curr Genet

Publication Date

12/1993

Volume

24

Pages

496 - 499

Keywords

Agaricus, Base Composition, Candida albicans, Cellulase, Chromosome Mapping, Chromosomes, Fungal, DNA, Fungal, DNA, Ribosomal, Electrophoresis, Agar Gel, Gene Rearrangement, Karyotyping, Laccase, Molecular Weight, Oxidoreductases, Saccharomyces cerevisiae, Schizosaccharomyces