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cdc28-1N is a conditional allele that has normal G1 (Start) function but confers a mitotic defect. We have isolated seven genes that in high dosage suppress the growth defect of cdc28-1N cells but not of Start-defective cdc28-4 cells. Three of these (CLB1, CLB2, and CLB4) encode proteins strongly homologous to G2-specific B-type cyclins. Another gene, CLB3, was cloned using PCR, CLB1 and CLB2 encode a pair of closely related proteins; CLB3 and CLB4 encode a second pair. Neither CLB1 nor CLB2 is essential; however, disruption of both is lethal and causes a mitotic defect. Furthermore, the double mutant cdc28-1N clb2::LEU2 is nonviable, whereas cdc28-4 clb2::LEU2 is viable, suggesting that the cdc28-1N protein may be defective in its interaction with B-type cyclins. Our results are consistent with CDC28 function being required in both G1 and mitosis. Its mitotic role, we believe, involves interaction with a family of at least four G2-specific cyclins.

Type

Journal article

Journal

Cell

Publication Date

05/04/1991

Volume

65

Pages

145 - 161

Keywords

Alleles, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cyclins, DNA, Fungal, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, Mitosis, Molecular Sequence Data, Mutation, Phenotype, Plasmids, Polymerase Chain Reaction, Protamine Kinase, Protein Kinases, Restriction Mapping, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid