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Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.

Type

Journal article

Journal

Science

Publication Date

15/11/1996

Volume

274

Pages

1201 - 1204

Keywords

Anaphase, Cell Cycle Proteins, Cell Division, Centrifugation, Density Gradient, Cyclins, Fluorescent Antibody Technique, Indirect, Fungal Proteins, G1 Phase, Genes, Fungal, Ligases, Mitosis, Saccharomyces cerevisiae, Ubiquitin-Protein Ligases, Ubiquitins