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Gene activation in eukaryotes requires chromatin remodeling complexes like Swi/Snf and histone acetylases like SAGA. How these factors are recruited to promoters is not yet understood. Using CHIP, we measured recruitment of Swi/Snf, SAGA, the repressor Ash1p, and transcription factors Swi5p and SBF to the HO endonuclease promoter as cells progress through the yeast cell cycle. Swi5p's entry into nuclei at the end of anaphase recruits Swi/Snf, which then recruits SAGA. These two factors then facilitate SBF's binding. Ash1p, which only accumulates in daughter cell nuclei, binds to HO soon after Swi5p and aborts recruitment of Swi/Snf, SAGA, and SBF. Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p.

Type

Journal article

Journal

Cell

Publication Date

30/04/1999

Volume

97

Pages

299 - 311

Keywords

Acetyltransferases, Cell Cycle, Cell Cycle Proteins, Chromatin, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Deoxyribonucleases, Type II Site-Specific, Drosophila Proteins, Fungal Proteins, Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Histone Acetyltransferases, Promoter Regions, Genetic, Protein Binding, RNA-Binding Proteins, Repressor Proteins, Ribonucleoprotein, U1 Small Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcriptional Activation, Zinc Fingers