Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Cell division in haploid yeast gives rise to a "mother" cell capable of mating-type switching and a "daughter" cell that is not. Switching is initiated by the HO endonuclease, whose gene is only transcribed in cells that have previously given birth to a bud (mother cells). HO expression depends on a minimyosin, She1p/Myo4p, which accumulates preferentially in growing buds. We describe a gene, ASH1, that is necessary to repress HO in daughters. ASH1 encodes a zinc finger protein whose preferential accumulation in daughter cell nuclei at the end of anaphase depends on She1p/Myo4p. The greater abundance of Ash1p in daughter cells is responsible for restricting HO expression to mother cells.

Type

Journal article

Journal

Cell

Publication Date

08/03/1996

Volume

84

Pages

699 - 709

Keywords

Amino Acid Sequence, Anaphase, Base Sequence, Cell Nucleus, Cloning, Molecular, Crosses, Genetic, DNA-Binding Proteins, Deoxyribonucleases, Type II Site-Specific, Epitopes, Fungal Proteins, Gene Expression, Genes, Fungal, Genes, Mating Type, Fungal, Molecular Sequence Data, Mutagenesis, Myosin Heavy Chains, Myosin Type V, Myosins, Open Reading Frames, Recombinant Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Species Specificity, Transcription Factors, Transcription, Genetic, Zinc Fingers