Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Activation of HO in yeast involves recruitment of transcription factors in two waves. The first is triggered by inactivation of Cdk1 at the end of mitosis, which promotes import into the nucleus of the Swi5 transcription factor. Swi5 recruits the Swi/Snf chromatin-remodeling complex, which then facilitates recruitment of the SAGA histone acetylase, which in turn permits the binding of the SBF transcription factor. We show here that SBF then recruits the SRB/mediator complex and that this process occurs in the absence of Cdk1 activity. The second wave is triggered by reactivation of Cdk1, which leads to recruitment of PolII, TFIIB, and TFIIH. RNA polymerase is, therefore, recruited to HO in two steps and not as a holoenzyme. A similar sequence of events occurs at other SBF-regulated promoters, such as CLN1, CLN2, and PCL1.

Type

Journal article

Journal

Mol Cell

Publication Date

06/2001

Volume

7

Pages

1213 - 1220

Keywords

CDC2 Protein Kinase, Cell Cycle, Cell Cycle Proteins, Cyclin-Dependent Kinases, DNA Polymerase III, DNA-Binding Proteins, DNA-Directed RNA Polymerases, Deoxyribonucleases, Type II Site-Specific, Fungal Proteins, Promoter Regions, Genetic, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, Transcription Factor TFIIB, Transcription Factor TFIID, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII, Yeasts