Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

BACKGROUND: Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression. RESULTS: We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Among these genes are several that are regulated by the steroid hormone ecdysone. Cytological visualization of transcription at selected ecdysone-responsive genes reveals that puffing at Eip74EF ceases within an hour or two of cohesin cleavage, long before any decline in ecdysone receptor could be detected at this locus. CONCLUSION: We conclude that cohesin regulates expression of a distinct set of genes, including those mediating the ecdysone response.

Original publication

DOI

10.1016/j.cub.2010.09.006

Type

Journal article

Journal

Curr Biol

Publication Date

26/10/2010

Volume

20

Pages

1787 - 1798

Keywords

Animals, Blotting, Western, Cell Cycle Proteins, Chromatids, Chromatin Immunoprecipitation, Chromosomal Proteins, Non-Histone, Chromosomal Puffs, Drosophila Proteins, Drosophila melanogaster, Ecdysone, Endopeptidases, Gene Expression Profiling, Gene Expression Regulation, Developmental, Salivary Glands