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In budding yeast genes that encode G1 cyclins and proteins involved in DNA synthesis are transcriptionally activated in late G1. A transcription factor, called SBF, is composed of Swi4 and Swi6 proteins and activates transcription of G1 cyclin genes. A different, but related, complex called MBF binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. MBF contains Swi6 and a 120-kilodalton protein (p120). MBF was purified and the gene encoding p120 (termed MBP1) was cloned. A deletion of MBP1 was not lethal but led to deregulated expression of DNA synthesis genes, indicating a direct regulatory role for MBF in MCB-driven transcription. Mbp1 is related to Swi4. Strains deleted for both MBP1 and SWI4 were inviable, demonstrating that transcriptional activation by MBF and SBF has an important role in the transition from G1 to S phase.

Type

Journal article

Journal

Science

Publication Date

17/09/1993

Volume

261

Pages

1551 - 1557

Keywords

Amino Acid Sequence, Base Sequence, CDC28 Protein Kinase, S cerevisiae, Cloning, Molecular, Cyclins, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, G1 Phase, Gene Expression Regulation, Fungal, Genes, Fungal, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors