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A multisubunit cohesin complex holds sister chromatids together after DNA replication. Using chromatin immunoprecipitation, we detected cohesin association with centromeres and with discrete sites along chromosome arms from S phase until metaphase in S. cerevisiae. Short DNA sequences (130-280 bp) are sufficient to confer cohesin association. Cohesin association with a centromere depends on Mif2p, the centromere binding factor CBF3, and a centromere-specific histone variant, Cse4p. Because only active centromeres confer cohesin association with centromeric DNA, we suggest that cohesin is recruited by the same chromatin structure that confers the attachment of microtubules. Propagation of this structure might be partly epigenetic. Finally, cohesion associated with "minimal" centromeres is insufficient to resist the splitting force exerted by microtubules and appears to be reinforced by cohesion provided by their flanking DNA sequences.

Type

Journal article

Journal

Cell

Publication Date

17/09/1999

Volume

98

Pages

847 - 858

Keywords

Binding Sites, Cell Cycle Proteins, Centromere, Chondroitin Sulfate Proteoglycans, Chromatids, Chromatin, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Histones, Kinetochores, Nuclear Proteins, Phosphoproteins, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins