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We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

Original publication

DOI

10.1016/j.celrep.2012.09.011

Type

Journal article

Journal

Cell Rep

Publication Date

25/10/2012

Volume

2

Pages

991 - 1001

Keywords

Animals, Animals, Genetically Modified, Brain, Databases, Factual, Drosophila Proteins, Drosophila melanogaster, Immunohistochemistry, Microscopy, Confocal, Nervous System, Transcription Factors, Transcription, Genetic