Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.
Methods Mol Biol
199 - 208
Behavior, Drosophila, In vivo models, Morphology analysis, Neurotoxicity, Animals, Animals, Genetically Modified, Behavior, Animal, Biological Assay, Biomarkers, Circadian Rhythm, Disease Models, Animal, Dopaminergic Neurons, Drosophila, Locomotion, Parkinson Disease, alpha-Synuclein