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We describe an efficient protocol for mapping genes and other DNA sequences to amphioxus chromosomes using fluorescent in situ hybridisation. We apply this method to identify the number and location of ribosomal DNA gene clusters and telomere sequences in metaphase spreads of Branchiostoma floridae. We also describe how the locations of two single copy genes can be mapped relative to each other, and demonstrate this by mapping an amphioxus Pax gene relative to a homologue of the Notch gene. These methods have great potential for performing comparative genomics between amphioxus and vertebrates.

Original publication

DOI

10.2108/zsj.19.1349

Type

Journal article

Journal

Zoolog Sci

Publication Date

12/2002

Volume

19

Pages

1349 - 1353

Keywords

Animals, Chordata, Nonvertebrate, Chromosomes, Color, DNA, Ribosomal, Genes, In Situ Hybridization, Fluorescence, Physical Chromosome Mapping, Telomere