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Class-switch variants have been isolated from rat-rat hybrid myelomas by sib selection using a simple assay based on red cell-labelled antiglobulins. The variants detected are consistent with the gene order deduced from molecular cloning. They appear to arise spontaneously at a rate approximately ten-fold lower than for mouse cell lines but the rate of switching back to the parental isotype is substantial in comparison. An IgG2b variant antibody having the same specificity as CAMPATH-1 for human lymphocytes and monocytes is active in antibody-dependent cell-mediated killing (unlike the parental IgG2a) and may prove to be a valuable therapeutic antibody for immunosuppression and treatment of leukaemia and lymphoma.

Type

Journal article

Journal

J Immunol Methods

Publication Date

23/10/1987

Volume

103

Pages

59 - 67

Keywords

Animals, Antibodies, Monoclonal, Antibody-Dependent Cell Cytotoxicity, Hemagglutination Tests, Hybridomas, Immunoglobulin G, Immunoglobulin Isotypes, Immunoglobulin Switch Region, Mice, Mutation, Rats, Tumor Cells, Cultured