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We have introduced a human beta-globin minilocus, containing the recently described dominant control region (DCR), the beta-globin or Thy-1 gene, and a thymidine kinase (tk)-neoR gene into erythroid and non-erythroid cells. Analysis of the transcription levels of the genes shows that the DCR directs high levels of human beta-globin, Thy-1 and tk-neo expression independent of integration sites in an erythroid-specific manner. The presence of the DNAasel hypersensitive sites at the 5' end of the locus is required for this effect on the homologous and heterologous gene. An analysis of the DCR chromatin in transfected mouse erythroleukemic cells suggests that the formation of the hypersensitive sites in this region precedes beta-globin gene expression.

Type

Journal article

Journal

Cell

Publication Date

24/03/1989

Volume

56

Pages

969 - 977

Keywords

Animals, Bone Marrow, Cell Line, Gene Expression Regulation, Genes, Dominant, Globins, Humans, Leukemia, Erythroblastic, Acute, Liver, Mice, Organ Specificity, Promoter Regions, Genetic, Transcription, Genetic