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A gene fusion plasmid was constructed by inserting the structural gene of the firefly luciferase into a vector which allows expression of a functional recombinant scFv. The fusion protein which is expressed in the periplasm of bacteria, retains dual activity and functions in bioluminescent immunoassays. Such a genetic approach offers many advantages over chemical cross-linking of antibodies to colorimetric enzymes and may be adaptable to clinical immunoassays and high through-put drug screening assays.

Original publication

DOI

10.1023/A:1018463804651

Type

Journal article

Journal

Biotechnology Letters

Publication Date

11/11/1997

Volume

19

Pages

1037 - 1041