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Plasmids containing sequences 3' of the adult beta 1 globin gene of Xenopus laevis are unstable on propagation in a range of E. coli host strains. Up to 300 bp of Xenopus DNA are lost by rec A independent recombination between (AT)37 and (AT)17 sequences. Additionally, smaller deletions occurring in or around the (AT)37 sequence are observed. Deletion of these potential cruciform structures occurs in the absence of exonuclease I, exonuclease V and exonuclease VIII as the same pattern of deletion events is observed in recA recBC sbcB and recBC sbcA recE strains.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

27/05/1986

Volume

14

Pages

4147 - 4158

Keywords

Animals, Base Sequence, Chromosome Deletion, DNA Restriction Enzymes, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Agar Gel, Escherichia coli, Globins, Nucleic Acid Hybridization, Plasmids, Recombination, Genetic, Xenopus laevis