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We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.

Original publication

DOI

10.1128/AEM.01587-09

Type

Journal article

Journal

Appl Environ Microbiol

Publication Date

10/2009

Volume

75

Pages

6613 - 6615

Keywords

Chromosome Mapping, Gene Expression, Genes, Bacterial, Genetic Vectors, Isopropyl Thiogalactoside, Kanamycin Resistance, Lac Operon, Molecular Sequence Data, Paracoccus denitrificans, Plasmids, Promoter Regions, Genetic, Replicon, Rhodobacter sphaeroides