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CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37 degrees C and 35 degrees C.

Type

Journal article

Journal

J Cell Sci

Publication Date

1993

Volume

105 ( Pt 2)

Pages

529 - 531

Keywords

Biological Markers CDC2 Protein Kinase/genetics/metabolism Carbon Dioxide/*metabolism Cell Cycle/drug effects Cell-Free System Cyclin B Cyclins/genetics/metabolism Energy Metabolism Fungal Proteins/genetics/metabolism Glucose/pharmacology Glycolysis Manometry Phenotype Schizosaccharomyces/genetics/growth & development/*metabolism