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Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-D-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions.

Type

Journal article

Journal

Neurosci Lett

Publication Date

29/08/1997

Volume

232

Pages

87 - 90

Keywords

Animals, Hippocampus, In Vitro Techniques, Ischemia, Male, Polymerase Chain Reaction, RNA, Messenger, Rats, Rats, Wistar, Receptors, N-Methyl-D-Aspartate, Time Factors