Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Replication of human immunodeficiency virus type 1 (HIV-1) is restricted to CD4-expressing primate cells. This tropism may be due partly to the absence from nonprimate cells of a species-specific factor which has an accessory role to CD4 during virus penetration. In this study we describe a rat B lymphocyte cell line in which there is efficient CD4-dependent entry of HIV-1. However, this cell line has a block to productive infection of HIV-1 at a stage between reverse transcription and integration. Our results demonstrate that the putative accessory factor for HIV-1 penetration is not restricted to primate cells and that there is a novel, uncharacterized cell-virus interaction at a stage between penetration and integration.

Original publication




Journal article


J Gen Virol

Publication Date



75 ( Pt 10)


2615 - 2623


Animals, Antibodies, Monoclonal, Antigens, CD, B-Lymphocytes, Base Sequence, CD4 Antigens, Cell Line, Cricetinae, DNA Primers, DNA, Viral, HIV Reverse Transcriptase, HIV-1, HeLa Cells, Humans, Kidney, Molecular Sequence Data, Multiple Myeloma, Polymerase Chain Reaction, RNA-Directed DNA Polymerase, Rats, Virus Integration, Virus Replication, Zidovudine