Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root Secretion.
Pini F., East AK., Appia-Ayme C., Tomek J., Karunakaran R., Mendoza-Suárez M., Edwards A., Terpolilli JJ., Roworth J., Downie JA., Poole PS.
Plants engineer the rhizosphere to their advantage by secreting various nutrients and secondary metabolites. Coupling transcriptomic and metabolomic analyses of the pea (Pisum sativum) rhizosphere, a suite of bioreporters has been developed inRhizobium leguminosarumbvviciaestrain 3841, and these detect metabolites secreted by roots in space and time. Fourteen bacterialluxfusion bioreporters, specific for sugars, polyols, amino acids, organic acids, or flavonoids, have been validated in vitro and in vivo. Using different bacterial mutants (nodCandnifH), the process of colonization and symbiosis has been analyzed, revealing compounds important in the different steps of the rhizobium-legume association. Dicarboxylates and sucrose are the main carbon sources within the nodules; in ineffective (nifH) nodules, particularly low levels of sucrose were observed, suggesting that plant sanctions affect carbon supply to nodules. In contrast, highmyo-inositol levels were observed prior to nodule formation and also innifHsenescent nodules. Amino acid biosensors showed different patterns: a γ-aminobutyrate biosensor was active only inside nodules, whereas the phenylalanine bioreporter showed a high signal also in the rhizosphere. The bioreporters were further validated in vetch (Vicia hirsuta), producing similar results. In addition, vetch exhibited a local increase ofnodgene-inducing flavonoids at sites where nodules developed subsequently. These bioreporters will be particularly helpful in understanding the dynamics of root exudation and the role of different molecules secreted into the rhizosphere.