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Activity-based probes are powerful tools to interrogate the functional proteome. Their covalent and often irreversible labeling of proteins facilitates the purification, identification and quantification of labeled proteins. However, the detection of labeled proteins often requires a confirmation, especially when unexpected proteins are identified, or to unravel fluorescent activity profiles. Here, we review twelve approaches towards target confirmation, grouped in approaches by direct target detection, target expression or target depletion. We discuss their proper use and limitations and illustrate these approaches with examples from plant science.

Original publication

DOI

10.1016/j.bmc.2016.05.036

Type

Journal article

Journal

Bioorg Med Chem

Publication Date

01/08/2016

Volume

24

Pages

3304 - 3311

Keywords

Activity-based protein profiling, Arabidopsis, Mass spectrometry, Probe targets, Tomato, Arabidopsis, Lycopersicon esculentum, Mass Spectrometry, Plant Proteins, Plants