Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

Type

Journal article

Journal

Opt Lett

Publication Date

15/10/2009

Volume

34

Pages

3154 - 3156

Keywords

Air, Animals, Cytoplasm, Developmental Biology, Embryo, Mammalian, Equipment Design, Glass, Image Processing, Computer-Assisted, Mice, Microscopy, Confocal, Models, Statistical, Optics and Photonics, Refractometry, Time Factors