Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Previously published data have shown an allele-specific variation in the in vitro binding of apolipoprotein E (apoE) to tau, which prompted the hypothesis that apoE binding may protect tau from phosphorylation, apoE3 being more efficient than apoE4. We have, therefore, investigated the effects of apoE on tau phosphorylation in vitro by the proline-directed kinase, glycogen synthase kinase (GSK)-3 beta. The phosphopeptide maps of tau alone, of tau with apoE3 and of tau with apoE4 were very similar. When apoE2 was present a further four spots were evident. Additionally, of the 15 peptides phosphorylated in the presence or absence of apoE, subtle differences, some isoform-specific, in the relative amounts of phosphorylation were observed.

Type

Journal article

Journal

FEBS Lett

Publication Date

24/11/2000

Volume

485

Pages

99 - 103

Keywords

Apolipoprotein E2, Apolipoprotein E3, Apolipoprotein E4, Apolipoproteins E, Calcium-Calmodulin-Dependent Protein Kinases, Glycogen Synthase Kinases, Humans, Peptide Mapping, Phosphoproteins, Phosphorylation, Recombinant Proteins, Spectrometry, Mass, Electrospray Ionization, Transfection, tau Proteins