Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

Original publication

DOI

10.3201/eid2204.151812

Type

Journal article

Journal

Emerg Infect Dis

Publication Date

04/2016

Volume

22

Pages

671 - 678

Keywords

Flaviviridae, HCV, HHpgV-1, Hepacivirus, Hepegivirus, Pegivirus, bloodborne pathogens, hemophilia, parenteral, persons who inject drugs, sexually transmitted disease, transfusion, virus persistence, viruses, Coinfection, Computational Biology, Factor VII, Flaviviridae, Flaviviridae Infections, Hemophilia A, Hepacivirus, High-Throughput Nucleotide Sequencing, Humans, Phylogeny, Polymerase Chain Reaction, Retrospective Studies, Sequence Analysis, DNA, Viremia