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In Drosophila oocytes, gurken/TGF-α mRNA is essential for establishing the future embryonic axes. gurken remains translationally silent during transport from its point of synthesis in nurse cells to its final destination in the oocyte, where it associates with the edge of processing bodies. Here we show that, in nurse cells, gurken is kept translationally silent by the lack of sufficient Orb/CPEB, its translational activator. Processing bodies in nurse cells have a similar protein complement and ultrastructure to those in the oocyte, but they markedly less Orb and do not associate with gurken mRNA. Ectopic expression of Orb in nurse cells at levels similar to the wild-type oocyte dorso-anterior corner at mid-oogenesis is sufficient to cause gurken mRNA to associate with processing bodies and translate prematurely. We propose that controlling the spatial distribution of translational activators is a fundamental mechanism for regulating localized translation.

Original publication

DOI

10.1016/j.celrep.2016.02.038

Type

Journal article

Journal

Cell Rep

Publication Date

15/03/2016

Volume

14

Pages

2451 - 2462

Keywords

Animals, DEAD-box RNA Helicases, Drosophila, Drosophila Proteins, Female, Gene Expression Regulation, Developmental, Genes, Reporter, In Situ Hybridization, Fluorescence, Microscopy, Fluorescence, Oocytes, Oogenesis, RNA, Messenger, RNA-Binding Proteins, Transforming Growth Factor alpha