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Konieczny and Ausubel have described a technique whereby Arabidopsis thaliana loci can be rapidly mapped to one of the ten chromosome arms using a small number of F2 progeny from crosses between the ecotypes Landsberg erecta and Columbia. The technique involves the use of 18 co-dominant, cleaved amplified polymorphic sequence (CAPS) markers which are evenly distributed throughout the Arabidopsis genome. We have mapped these 18 markers using recombinant inbred (RI) lines generated in our laboratory. These data enable a better integration of loci mapped relative to the CAPS markers into the restriction fragment length polymorphism (RFLP) map generated using Arabidopsis RI lines.

Type

Journal article

Journal

Plant Mol Biol

Publication Date

02/1994

Volume

24

Pages

685 - 687

Keywords

Arabidopsis, Breeding, Genes, Plant, Genetic Markers, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length