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The atypical myrosinase PENETRATION2 (PEN2) is required for broad-spectrum invasion resistance to filamentous plant pathogens. Previous localization studies suggested PEN2-GFP association with peroxisomes. Here, we show that PEN2 is a tail-anchored protein with dual-membrane targeting to peroxisomes and mitochondria and that PEN2 has the capacity to form homo-oligomer complexes. We demonstrate pathogen-induced recruitment and immobilization of mitochondrial subpopulations at sites of attempted fungal invasion and show that mitochondrial arrest is accompanied by peripheral accumulation of GFP-tagged PEN2. PEN2 substrate production by the cytochrome P450 monooxygenase CYP81F2 is localized to the surface of the endoplasmic reticulum, which focally reorganizes close to the immobilized mitochondria. Exclusive targeting of PEN2 to the outer membrane of mitochondria complements the pen2 mutant phenotype, corroborating the functional importance of the mitochondrial PEN2 protein subpool for controlled local production of PEN2 hydrolysis products at subcellular plant-microbe interaction domains. Moreover, live-cell imaging shows that mitochondria arrested at these domains exhibit a pathogen-induced redox imbalance, which may lead to the production of intracellular signals.

Original publication

DOI

10.1105/tpc.15.00887

Type

Journal article

Journal

Plant Cell

Publication Date

01/2016

Volume

28

Pages

130 - 145

Keywords

Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Ascomycota, Disease Resistance, Endoplasmic Reticulum, Green Fluorescent Proteins, Host-Pathogen Interactions, Mitochondria, Mitochondrial Membranes, Molecular Sequence Data, N-Glycosyl Hydrolases, Oxidation-Reduction, Peroxisomes, Plant Diseases, Plant Epidermis, Plant Leaves, Protein Multimerization, Subcellular Fractions, Substrate Specificity