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Chromosome-specific paint probes provide a powerful tool with wide applications in cytogenetic analysis. Here, we present a new approach using UV-laser microbeam microdissection in combination with laser-pressure catapulting, which allows the fast isolation of single chromosomes for the generation of chromosome-specific paint probes. To demonstrate the feasibility of this approach, single chromosomes were collected and amplified with degenerate oligonucleotide-primed PCR, hapten-labeled and hybridized onto normal metaphase spreads. Fluorescence in situ hybridization signals revealed specific painting of the respective chromosomes.

Type

Journal article

Journal

Biotechniques

Publication Date

08/1999

Volume

27

Pages

362 - 367

Keywords

Animals, Cells, Cultured, Chromosome Painting, Cricetinae, DNA Probes, In Situ Hybridization, Fluorescence, Lasers, Polymerase Chain Reaction, Ultraviolet Rays